November 18, 2020

Ribosome profiling strategy

Reverse transcription with TSO works

rRNA targeted DSN treatment works

Amplification works

Size discrepancy: Faulty bioanalyzer or complex formation?

Previous strategy, time to move back?

Moving forward

  1. Order reverse transcription primer from previous strategy (costs minimally and we can be sure that the library prep will work)

  2. Sequence new strategy on MiSeq (I don’t trust the BioAnalyzer)